Daniel Wagenaar Thesis

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We hypothesized that synchronized bursting can dominate activity patterns, because lack of external input puts cultures in a hypersensitive state.Update (August 2019): Both positions have been filled.(Regardless, exceptional self-funded candidates will still be considered.) We are looking for up to two postdoctoral scholars to work on reconstructing the connectome of leech ganglia from which we have recorded the activity of all the neurons during a variety of behaviors.A position is also available for a highly qualified and motivated graduate student. The detailed protocol for the experiments in Yusuke Tomina’s recent e Life paper appeared today in Bio Protocol.(The full text and videos can be downloaded without creating an account from my Publications page.) Drew Lehmkuhl and Arun Muthusamy’s joint first-author paper on neuronal responses to sensory stimuli from water waves appeared in the Journal of Experimental Biology. Funding for the lab is provided by the National Institute of Neurological Disorders and Stroke through grant R01NS094403 and by the California Institute of Technology.By growing cultures on multi-electrode arrays (Petri dishes with a grid of substrate-embedded electrodes), their electrical activity can be recorded non-invasively.I developed software, MEABench, for online visualization and analysis of multi-electrode data, and used it to follow the development of cultures obtained from (E18) embryonic rats.Past funding came from the Burroughs Wellcome Fund in the form of a Career Award at the Scientific Interface, from the University of Cincinnati and from The Broad Foundations.All support, past and present, is gratefully acknowledged.The number of bursts within these 'superbursts' and their spatiotemporal structure were found to be stable for hours or days.Cortical cultures on multi-electrode arrays are ideal for studying two-way communication between biological systems and computers.


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